Coding

Part:BBa_K2593003

Designed by: Huimin Li   Group: iGEM18_SSTi-SZGD   (2018-09-20)


AmyX signal peptide is encoded by a-Amylase gene which is derived from B.amyloliquefaciens.

a-Amylase contains a signal peptide (31 amino acid long) at the N-terminus of the domain chain to facilitate the exportation of the protein. AmyX signal peptide belongs to the twin-arginine translocation (Tat) pathway of the Bacillus origin. The Tat pathway has a signature twin-arginine (RR) motif located at the border of the N-terminal domain and the hydrophobic region. Gram-positive bacteria usually contain only TatAC subunits. In Bacillus subtilis, there are two TatAC-type systems, TatAdCd and TatAyCy, operate in parallel with different substrate specificities. in our project AmyX was fused in N-terminal of the LHyal gene for the extracellular export of the expressed leech hyaluronidase.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Reference

Sunghoon Park, Wolfgang Schumann. Optimization of the Secretion Pathway for Heterologous Proteins in Bacillus subtilis[J].Biotechnology and Bioprocess Engineering,2015:626-627

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